SIGNALING PATHWAYS INVOLVED IN MULTIPLE MYELOMA-BONE MARROW STROMAL CELL INTERACTION

Project funded by THE NATIONAL UNIVERSITY RESEARCH COUNCIL, Ministry of Education and Research, Romania (contract no. 395, 01-10-2007)
Infrastructure
En | Ro

Research infrastructure available for project implementation:
Flow cytometer with 2 lasers FACS Calibur
EPICS X lflow cytometer (Coulter)

Biological probes /cell cultures :
Sterile Laminar Airflow Hoods :Holten model HBB 2472, Holten S2000 model 1.8, TelStar Bio II A/G
CaptAir fume hood with special filters for organic solvents
2xCO2 incubator (Jouan IG150), CO2 incubator (Revco Habitat), CO2 incubator Sanyo MCO-15AC
Sterilization : Horizontal 50 L autoclav (Raypa Model AH-75-DRY), 3x Memmert ovens

Cell separation equipments, centrifugation :
Centrifuges :Jouan MR 18-22 cooling centrifuge, Jouan CR 4-22 cooling centrifuge, Hettich Micro 20 centrifuge, Hettich cyto-centrifuge (Model Universal 32R), Selecta Mixtasel centrifuge
Tissue Mecanical Disaggregation Systems (Medimachine, Dako, Medimachine, Becton Dickinson)
Magnetic Separator MACS (Becton-Dickinson)

Molecular Biology Equipment:
Immunobloting system (Biorad, Western Processor) with 3 cell transfer trays (Model Mini-Protean)
Pulsed field electrophoresis, CHEF DR III System (including electrophoresis cell, variable speed pump, cooling module), Gel dryer (Biorad, Model 538) Gel Dryer with Hydrotech vacuum pump
2x BioRad GelDoc 2000 digital gel documentation systems
Bio-Rad imaging system (FLUOR-S MultiImager), for capturing digital images from fluorescence, chemiluminescence, chemifluorescence, and colorimetric samples

Cryopreservation: -80˚C freezer (Jouan Model VX530), Liquid nytrogen cryopreservation system (AirLiquide TP100), with Dewar tanks for nytrogen transport and storage, -20˚C freezers and 4˚C refrigerators

General:
3x De-ionising and filtering water systems (TKA model Lab-UPW, Millipore MilliRO10 plus, and MilliQ UF plus)
Analitical Balance, 0.1mg – 99g (Sartorius), Analitical Balance, 0.1mg – 210g (A&D, Model HM-200)
Balance, 0.5 – 1250g (A&D, Model HF-1200G)
pH-meter (Inolab, Model Level 2), pH-meter (Hana), Spectrophotometer (Pharmacia LKB Ultrospec Plus)
Orbital shaker for 4 plates of 96 wells (Heidolph Instruments Model Titramax 100), Tube vortexes
2x magnetic homogenizers (Heidolph MR1000 and Sigma)
3x Water bath (Jouan, JT30B Heto, Model AG, and WB7 Memmert), Shakers (MIX10 and MIX20)

Created by Dragoş & Costel
Last update: Monday, April 26, 2010 20:59